Identification and Characterization of a Monofunctional Glycosyltransferase from Staphylococcus aureus
نویسندگان
چکیده
منابع مشابه
Kinetic characterization of the monofunctional glycosyltransferase from Staphylococcus aureus.
The glycosyltransferase (GT) module of class A penicillin-binding proteins (PBPs) and monofunctional GTs (MGTs) belong to the GT51 family in the sequence-based classification of GTs. They both possess five conserved motifs and use lipid II precursor (undecaprenyl-pyrophosphate-N-acetylglucosaminyl-N-acetylmuramoyl- pentapeptide) to synthesize the glycan chain of the bacterial wall peptidoglycan...
متن کاملKinetic characterization of the glycosyltransferase module of Staphylococcus aureus PBP2.
We report the heterologous overexpression and purification of Staphylococcus aureus PBP2 and demonstrate efficient glycan polymerization from lipid II in vitro. S. aureus PBP2 is the first purified gram-positive class A penicillin-binding protein to show good transglycosylase activity. This enables further studies on this important class of enzymes.
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Objective(s) Staphylococcus aureus is a common cause of human infection, and emergence of vancomycin-resistance S. aureus is a great concern for treatment of methicillin-resistant S. aureus,(MRSA) in recent years (MRSA). Here, we report the isolation of high-level VRSA. Materials and Methods S. aureus was isolated from foot ulcer of a diabetic woman in Tehran, Iran. Antibiotic susceptibilit...
متن کاملIdentification and characterization of the pckA gene from Staphylococcus aureus.
The Staphylococcus aureus pckA gene was identified and characterized. A pckA mutant lacked detectable phosphoenolpyruvate carboxykinase activity and grew poorly in the absence of glucose. Both enzymatic activity and pckA promoter activity in wild-type cells grown in the absence of glucose were at least 22-fold greater than activities in cells grown in the presence of glucose.
متن کاملidentification and characterization of a high vancomycin-resistant staphylococcus aureus harboring vana gene cluster isolated from diabetic foot ulcer
objective(s) staphylococcus aureus is a common cause of human infection, and emergence of vancomycin-resistance s. aureus is a great concern for treatment of methicillin-resistant s. aureus,(mrsa) in recent years (mrsa). here, we report the isolation of high-level vrsa. materials and methods s. aureus was isolated from foot ulcer of a diabetic woman in tehran, iran. antibiotic susceptibility w...
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ژورنال
عنوان ژورنال: Journal of Bacteriology
سال: 2001
ISSN: 0021-9193,1098-5530
DOI: 10.1128/jb.183.16.4779-4785.2001